Figure 7. Two copies of sry-α are required for reliable cellularization at the extreme temperature of 32°C or when the embryo is challenged by a genetic perturbation.

(A, C) Frequency of multinucleation for heterozygous embryos reared (A) at 32°C, or (C) with a reduced dose of maternal Profilin (½profilin; mean ± s.e.m.). (B, D) Quantification of multinucleation phenotype for heterozygous embryos reared (B) at 32°C, or (D) with a reduced dose of maternal Profilin (½profilin). Each point represents one embryo with ≥150 nuclei analyzed (n = 23 embryos per condition). Two-way ANOVA analysis was performed to calculate P values as shown in (A–D).