Table 1. Strains.
Strains | Genotype* | Source (ref.) |
AB1157 | thr-1, araC14, leuB6, lacY1, tsx-33, qsr'-0, glnV44, galK2, LAM-, Rac-0, hisG4, rfbC1, mgl-51, rpoS396, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3, thi-1 | M. Pato |
MP1999 | AB1157, recB, recC, sbcB, malF::Mucts62 | M. Pato |
MG1655 | F− λ− ilvG − rfb-50 rph-1 | Lab stock |
EC1512 | argE thi ilv gyrB402 | Filutowicz (1983) |
SIMD30 | W3110 lacDU169 galKTYR145UAG (λc1857Δcro-bioA) [l int'::cat-sacB] | Datta (2008) |
ZL524 | MP1999, loxP sites inserted 61 bp and 70 bp outside attL and attR, respectively | This study |
ZL530 | ZL524, Mucts B::kan | This study |
ZL536 | ZL524, Mucts A::kan | This study |
ZL552 | ZL524, Mucts ΔattL | This study |
ZL556 | ZL524, Mucts ΔattR | This study |
ZL562 | ZL524, Mucts ΔSGS | This study |
ZL573 | ZL562, Mucts gp23 ::SGS:: gp24 (SGS at 11.4 kb from L end) | This study |
ZL 578 | ZL562, Mucts gp41::SGS (SGS at 24 kb from L end) | This study |
ZL580 | MP1999 ΔMu, malF::loxP1 | This study |
ZL 582 | ZL580, malF::loxP2 (2nd site next to loxP1) | This study |
ZL592 | ZL580, yjcF::loxP (37 kb downstream of malF) | This study |
ZL594 | ZL580, purH::loxP (37 kb upstream of malF) | This study |
ZL598 | ZL592, pspG::SGS | This study |
ZL604 | ZL524, himA::kan | This study |
ZL614 | ZL524, fis::kan | This study |
ZL624 | ZL524, hns::kan | This study |
ZL634 | ZL524, hupA::kan | This study |
ZL652 | ZL524, deletion of 74 bp spacer between L1 and L2 sites within Mu attL | This study |
ZL656 | ZL524, Mucts Δ IHF site | This study |
ZL660 | ZL524, Mucts Δsis | This study |
ZL662 | ZL660, Mucts ΔattR | This study |
ZL670 | ZL524, Mucts ΔPmom | This study |
ZL672 | ZL662, Mucts ΔPmom | This study |
ZL704 | ZL580, lamB::loxP (5 kb downstream of malF) | This study |
ZL706 | ZL704, malE::SGS | This study |
ZL708 | ZL580, ubiC::loxP (9 kb downstream of malF) | This study |
ZL710 | ZL708, lamB:: SGS | This study |
ZL810 | ZL580, dinF::loxP::yjbJ (15 kb downstream of malF) | This study |
ZL712 | ZL580, yjbS::loxP::aphA (25 kb downstream of malF) | This study |
ZL714 | ZL712, plsB::SGS | This study |
ZL720 | MP1999, lamB::loxP, yjbH::loxP | This study |
ZL724 | MP1999, ubiC::loxP, lysC::loxP::pgi | This study |
ZL728 | MP1999, yjbS::loxP::aphA, aceA::loxP::aceK | This study |
ZL732 | MP1999, Mucts E7:: loxP, gp49::loxP | This study |
ZL808 | MG1655, λcI857Cm, loxP sites inserted 80 bp and 121 bp outside λ attL and attR, respectively | This study |
ZL901 | MG1655, lacZ::Muc+ (between 366204–366205 nt) | This study |
ZL911 | ZL901, loxP sites inserted 50 bp and 60 bp outside Muc+ attL and attR, respectively | This study |
ZL921 | ZL911, Muc+ ΔSGS | This study |
ZL931 | ZL911, hns::kan | This study |
ZL936 | ZL911, himA::kan | This study |
ZL940 | EC1512, yidX::cat-sacB | This study |
ZL941 | ZL911, yidX::cat-sacB gyrB402 | This study |
ZL951 | ZL524, Mu c::kan ΔPe and ΔPe* | This study |
RS005 | ZL524, Mucts Δ10 kb left arm of Mu | This study |
RS020 | RS005, Mucts Δ10 kb right arm of Mu | This study |
RS025 | RS020, Mucts ΔSGS | This study |
RS059 | ZL580, yjbY::loxP (17 kb downstream of malF) | This study |
RS053 | ZL524, Mucts ΔPe | This study |
RS092 | ZL524, Mucts ΔPe* | This study |
RS093 | ZL524, Mucts ΔPe ΔPe* | This study |
RS033 | MP1999, Mucts B::egfp-B | This study |
RS088 | RS033, Mucts ΔSGS | This study |
RS048 | RS033, Mucts ΔPe | This study |
RS102 | RS033, Mucts ΔPe* | This study |
RS103 | RS033, Mucts ΔPe ΔPe* | This study |
RS101 | MP1999, Mucts E::egfp-E | This study |
RS106 | RS048, Mucts SGS::kan-ccdB | This study |
RS107 | RS102, Mucts SGS::kan-ccdB | This study |
Δ, deletion of genes/sites.
:: indicates deletion-substitution when an antibiotic resistance cassette or EGFP is inserted in the indicated gene, but insertion when a site (loxP/SGS) or Mu is inserted. :: placed on both sides of a site indicates insertion of that site between the neighboring genes, the exact location given in Table S1.