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. 2013 Nov 7;9(11):e1003902. doi: 10.1371/journal.pgen.1003902

Table 1. Strains.

Strains Genotype* Source (ref.)
AB1157 thr-1, araC14, leuB6, lacY1, tsx-33, qsr'-0, glnV44, galK2, LAM-, Rac-0, hisG4, rfbC1, mgl-51, rpoS396, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3, thi-1 M. Pato
MP1999 AB1157, recB, recC, sbcB, malF::Mucts62 M. Pato
MG1655 F λ ilvG rfb-50 rph-1 Lab stock
EC1512 argE thi ilv gyrB402 Filutowicz (1983)
SIMD30 W3110 lacDU169 galKTYR145UAG (λc1857Δcro-bioA) [l int'::cat-sacB] Datta (2008)
ZL524 MP1999, loxP sites inserted 61 bp and 70 bp outside attL and attR, respectively This study
ZL530 ZL524, Mucts B::kan This study
ZL536 ZL524, Mucts A::kan This study
ZL552 ZL524, Mucts ΔattL This study
ZL556 ZL524, Mucts ΔattR This study
ZL562 ZL524, Mucts ΔSGS This study
ZL573 ZL562, Mucts gp23 ::SGS:: gp24 (SGS at 11.4 kb from L end) This study
ZL 578 ZL562, Mucts gp41::SGS (SGS at 24 kb from L end) This study
ZL580 MP1999 ΔMu, malF::loxP1 This study
ZL 582 ZL580, malF::loxP2 (2nd site next to loxP1) This study
ZL592 ZL580, yjcF::loxP (37 kb downstream of malF) This study
ZL594 ZL580, purH::loxP (37 kb upstream of malF) This study
ZL598 ZL592, pspG::SGS This study
ZL604 ZL524, himA::kan This study
ZL614 ZL524, fis::kan This study
ZL624 ZL524, hns::kan This study
ZL634 ZL524, hupA::kan This study
ZL652 ZL524, deletion of 74 bp spacer between L1 and L2 sites within Mu attL This study
ZL656 ZL524, Mucts Δ IHF site This study
ZL660 ZL524, Mucts Δsis This study
ZL662 ZL660, Mucts ΔattR This study
ZL670 ZL524, Mucts ΔPmom This study
ZL672 ZL662, Mucts ΔPmom This study
ZL704 ZL580, lamB::loxP (5 kb downstream of malF) This study
ZL706 ZL704, malE::SGS This study
ZL708 ZL580, ubiC::loxP (9 kb downstream of malF) This study
ZL710 ZL708, lamB:: SGS This study
ZL810 ZL580, dinF::loxP::yjbJ (15 kb downstream of malF) This study
ZL712 ZL580, yjbS::loxP::aphA (25 kb downstream of malF) This study
ZL714 ZL712, plsB::SGS This study
ZL720 MP1999, lamB::loxP, yjbH::loxP This study
ZL724 MP1999, ubiC::loxP, lysC::loxP::pgi This study
ZL728 MP1999, yjbS::loxP::aphA, aceA::loxP::aceK This study
ZL732 MP1999, Mucts E7:: loxP, gp49::loxP This study
ZL808 MG1655, λcI857Cm, loxP sites inserted 80 bp and 121 bp outside λ attL and attR, respectively This study
ZL901 MG1655, lacZ::Muc+ (between 366204–366205 nt) This study
ZL911 ZL901, loxP sites inserted 50 bp and 60 bp outside Muc+ attL and attR, respectively This study
ZL921 ZL911, Muc+ ΔSGS This study
ZL931 ZL911, hns::kan This study
ZL936 ZL911, himA::kan This study
ZL940 EC1512, yidX::cat-sacB This study
ZL941 ZL911, yidX::cat-sacB gyrB402 This study
ZL951 ZL524, Mu c::kan ΔPe and ΔPe* This study
RS005 ZL524, Mucts Δ10 kb left arm of Mu This study
RS020 RS005, Mucts Δ10 kb right arm of Mu This study
RS025 RS020, Mucts ΔSGS This study
RS059 ZL580, yjbY::loxP (17 kb downstream of malF) This study
RS053 ZL524, Mucts ΔPe This study
RS092 ZL524, Mucts ΔPe* This study
RS093 ZL524, Mucts ΔPe ΔPe* This study
RS033 MP1999, Mucts B::egfp-B This study
RS088 RS033, Mucts ΔSGS This study
RS048 RS033, Mucts ΔPe This study
RS102 RS033, Mucts ΔPe* This study
RS103 RS033, Mucts ΔPe ΔPe* This study
RS101 MP1999, Mucts E::egfp-E This study
RS106 RS048, Mucts SGS::kan-ccdB This study
RS107 RS102, Mucts SGS::kan-ccdB This study
*

Δ, deletion of genes/sites.

:: indicates deletion-substitution when an antibiotic resistance cassette or EGFP is inserted in the indicated gene, but insertion when a site (loxP/SGS) or Mu is inserted. :: placed on both sides of a site indicates insertion of that site between the neighboring genes, the exact location given in Table S1.