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. 2013 Nov 7;9(11):e1003903. doi: 10.1371/journal.pgen.1003903

Figure 2. RNAi knockdown of His2Av function in early germ cells results in GSC loss.

Figure 2

(A–D): Phase images of testes with RNAi knockdown of His2Av in GSCs and early germ cells using the NG4VP16 driver (B, D) and sibling control (A, C) at day 4 (A, B), day 12 (D) and day 15 (C) after RNAi induction. Arrows point to elongated spermatids. Scale Bars: 50 µm (E–H): Apical tips of testes with RNAi knockdown of His2Av in the germline (F, H) and sibling control (E, G) at day 0 (E, F) and day 12 (G, H) after RNAi induction and immunostained with anti-Vasa (red), anti-Fas3 (blue), and DAPI (green); hub (yellow dashed line). Scale bars (A–E): 10 µm(I): Quantification of GSC number over days after His2Av RNAi induction (red line) and sibling control (blue line). Data shows average ± S.D.