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. 2013 Nov 7;9(11):e1003961. doi: 10.1371/journal.pgen.1003961

Figure 5. Synergistic effects of vps-52 on the abundance of mature let-7 family miRNAs.

Figure 5

(A, C) The precursor and mature forms of miR-48 (A) and miR-241 (C) were investigated by Northern blotting in mid-L3 synchronized animals. The tRNAGly was used as control RNA. (B, D) The abundance of the miR-48 (B) and miR-241 (D). miRNAs level was measured by quantitative real-time PCR (TaqMan assay) in mid-L3 synchronized mutant animals and compared with the level found in wild type worms (WT: 1). The small nucleolar RNAs sn2841 was used as the normalization control. The error bars represent the standard deviation of three independent experiments and p values were obtained using a two-sided Student t-test.