Table 2. Effect of replication direction relative to transcription on mutation rates.
Mutation rate (×10−10) | |||||
lys2 allele | Relevant | 2- or-4 bp deletions | QP mutations | ||
genotype | SAME | OPPO | SAME | OPPO | |
lys2ΔBgl | WT | 10.2 | 9.7 | <10* | <9.7* |
rnh201Δ | 1160 | 1500 | 865 | <29* | |
rnh201Δ top1Δ | <23* | <19* | 631 | <19** | |
lys2ΔA746 | WT | 62.2 | 54.1 | <3.7* | <3.6* |
rnh201Δ | 1220 | 689 | 499 | 48 | |
rnh201Δ top1Δ | <16.3* | 7.8 | 326 | 31 | |
lys2ΔA746,NR | WT | <1.9* | <1.6* | <1.9* | <1.6* |
rnh201Δ | 796 | 394 | 337 | <12* | |
rnh201Δ top1Δ | <18* | <8.9* | 338 | 36 |
All rates were measured under high-transcription conditions. Total Lys+ rates and the associated 95% confidence intervals are provided in Supplemental Tables.
No events were observed; rate calculated assuming one event.