FIGURE 5.
Desensitization of mutants derived from ETA-4PD. HEK-293 cells were transiently transfected with constructs of ETA-WT or mutants as indicated. Cells were incubated for 2 h in the absence (basal) or presence of 100 nm ET-1. IP1 accumulation was quantified as described under “Experimental Procedures,” and values above the basal level were normalized to the value above the basal level of ETA-6PD. Bars represent the mean ± S.D. of two independent experiments, each performed at least in triplicate. Data were compared with the values of ETA-6PD (*, p < 0.001), ETA-5PD (#, p < 0.01), or ETA-WT ($, p < 0.001) using one-way ANOVA with Bonferroni correction.