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SA IL-1α is localized to the nucleus in a redox- and calpain-dependent fashion. A–E, confocal microscopy of a DsRed-IL-1α construct 16 h post-transient transfection into IMR-90 fibroblasts passaged as indicated at 21 or 3% O2 tension. Catalase (Cat) was added exogenously at 500 units/ml 3 h post-transfection. The calpain inhibitor (Calp) was used at a concentration of 40 μm and was added to the medium 3 h post-transfection. Data represent n ≥ 3.
