FIGURE 4.

In contrast to Ifitm3 N termini, the C termini are mostly or entirely extracellular. MEFs expressing either Myc-Ifitm3 or Ifitm3-C9 were first fixed and then either permeabilized with detergent or left with membranes intact prior to indirect immunofluorescence. Ifitm3 epitope tag staining is shown in green (top panels). Membrane integrity was assessed by co-staining for α-tubulin (middle panels, red). Nuclei were counterstained with DAPI (blue). Exposure times for each channel were identical, and contrast adjustment was applied uniformly to all panels for each color channel.