FIGURE 5.

The addition of a C-terminal KDEL motif to Myc-Ifitm3 results in its retention in the ER. Top panels show Myc (N-terminal) staining of cells expressing Ifitm3 with or without the indicated C-terminal additions. Middle panels show staining for FLAG-tagged Sptlc1 (an ER marker). Myc-Ifitm3-KDEL was poorly expressed in comparison with the other constructs, so the brightness of this panel was adjusted independently to better visualize its co-localization with Sptlc1. The nucleolar Myc staining is an artifact of the 9E11 antibody and has been previously described (17).