FIGURE 7.

C-terminal tags do not impair the restriction activity or expression of Ifitm3. A, MEFs were transduced with a vector control or the indicated Ifitm3 constructs. Transduced MEFs were then infected with eGFP-encoding retroviruses pseudotyped with the envelope glycoproteins of four different viruses. Bars show the proportion of the percentage of infected (eGFP⁺) cells expressing each Ifitm3 construct relative to percentage of infected vector-transduced cells infected. Error bars show the median and range of triplicate wells. VSV, vesicular stomatitis virus; LCMV, lymphocytic choriomeningitis virus. B, expression levels of the constructs were determined by Western blot against the native Ifitm3 N terminus.