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Substitution of TWEAK Tyr176 does not significantly affect TWEAK structure or surface charge. IVTT lysates containing FLAG epitope-tagged sTWEAK, sTWEAK Y176A, or sTWEAK Y176F were resolved by native gel electrophoresis. IVTT lysate with GFP cDNA as template was run as negative control. Lysates were immunoblotted with anti-FLAG monoclonal antibody.
