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. 2013 Sep 20;288(45):32261–32276. doi: 10.1074/jbc.M113.493536

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Substitution of TWEAK Tyr¹⁷⁶ disrupts Fn14-TWEAK interaction. Top, immunoblot analysis of IVTT lysates using anti-FLAG antibody showing equal expression of sTWEAK and sTWEAK Tyr¹⁷⁶ variants. Bottom, double sandwich ELISA depicting binding of sTWEAK and TWEAK Tyr¹⁷⁶ variants to Fn14. sTWEAK shows significantly higher binding as compared with no DNA lysate control, and TWEAK Tyr¹⁷⁶ variants show significantly lower binding as compared with sTWEAK (**, p < 0.001).