FIGURE 9.

The CROLIN1 loss-of-function mutation results in pollen germination and pollen tube growth hypersensitive to Lat B. A, genetic map of the Arabidopsis CROLIN1 gene. CROLIN1 contains five exons and four introns, which are indicated by filled boxes and lines, respectively. The position of the T-DNA insertion in mutant crolin1 (SAIK_108507) is indicated by triangles above the diagram. The insertion site is between bp 138 and 139, upstream from the ATG codon. The transcript levels in WT, crolin1, and the complemented line were determined by real-time PCR using EF1A as the internal control. Shown is the mean ± S.E. (error bars) of three biological replicates. **, p < 0.01 (Student's t test). B, germination of pollen grains from WT, crolin1, and the complemented line. The concentrations of Lat B used in this analysis were 0, 1, 1.5, and 2 nm. Error bars, S.E. (n ≥ 300). C, relative growth rate of the pollen tubes from WT, crolin1, and the complemented line. The concentrations of Lat B used in this analysis were 0, 2, 4, and 6 nm. Error bars, S.E. (n ≥ 150). D, pollen tube deformity rates of WT, crolin1, and the complemented line in the presence of 0, 2, 4, and 6 nm Lat B. Error bars, S.E. (n ≥ 150). The inset shows the deformity types. The main pollen tube deformities in the crolin1 mutant in the presence of Lat B are shown. a, normal pollen tube; b, enlargement; c, turns. Bars, 10 μm.