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The effect of soluble Gln1238–Glu1260 on GPIbα interactions with VWF and two forms of A1. A–C, plots of lifetime versus force of platelet lysate GPIbα bonds with VWF (A), 1238-A1 (B), or 1261-A1 (C) in the presence of soluble Lp or Sp (10 μg/ml or 4.2 μm). Lifetimes (mean ± S.E. of >20 measurements per point) were measured by the thermal fluctuation assay (closed symbols) at zero force and by the force clamp assay at nonzero forces (open symbols).
