FIGURE 3.

Effect of BSA and ferritin on the de novo fatty acid mixture promoted amyloid fibril formation of β2m. a, serum fatty acid mixture (250 μm) promoted de novo amyloid fibril formation of β2m alone at pH 7.5, monitored by ThT fluorescence, (■), in the presence of BSA (●), and in the presence of ferritin (▴). Molar ratios of β2m/BSA and β2m/ferritin were 1:0.30. Shown are transmission electron microscope images of the sample of β2m (plus 250 μm fatty acid mixture) incubated in the presence of BSA (b) and ferritin (c) at a molar ratio of 1:0.30. Scale bars in b and c, 200 nm. a.u., arbitrary units.