TABLE 1.

Apparent kinetic parameters for phosphorolysis and reverse phosphorolysis reactions catalyzed by UhgbMP, calculated from three to five independent experiments

Substrate concentrations were as follows: phosphorolysis, 10 mm inorganic phosphate and 1–20 mm β-d-mannopyranosyl-1,4-d-mannose; 1–10 mm β-d-mannopyranosyl-1,4-d-glucose, 0.4–4 mmβ-1,4-d-mannan, or 0.1–1mm pNP-β-d-mannopyranose, and 5 mm inorganic phosphate and 0.05–0.5 mm β-d-mannopyranosyl-1,4-N,N′-diacetylchitobiose; reverse phosphorolysis, 10 mm α-d-mannopyranose 1-phosphate and 5–40 mm d-glucose, d-mannose, d-galactose, d-fructose, or N-acetyl-d-glucosamine, and 5 mm α-d-mannopyranose 1-phosphate and 0.1–1 mm N,N′-diacetylchitobiose.