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Na+ kinetics of Asp91 mutants. COS-1 cells transfected with WT_ASBT and mutants of Asp91 were incubated with 5 μm cold TCA spiked with 1 μCi/ml [3H]TCA under sodium concentrations ranging from 0 to 137 mm at 37 °C for 12 min. Changes in sodium concentration were compensated with choline chloride.
