FIGURE 4.

A gas chromatography/mass spectrometry analysis of FAMEs is shown using analysis method 1 of yeast expressing SaFAD2 (A) or yeast expressing the empty vector pYES2 with no added substrates (B). Shown is yeast expressing SaFAD2 (C) and empty vector pYES2 (D) where both samples were supplemented with stearolic acid (C18:1 9A) during gene expression and an extract of S. acuminatum seed oil (E). In C, the product designated with an asterisk was identified as trans-12-octadecen-9-ynoic acid, but the product denoted as U was not conclusively identified. Fatty acids are represented by XX:YΔZ where XX is the carbon chain length, Y is the total number of carbon-carbon double or triple bonds in the molecule, and Z is the position of insertion of the unsaturation. A indicates an acetylenic bond. New products formed in the sample are indicated by underlined letters. Results are shown for the INVSc1 yeast strain.