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. 2013 Sep 24;288(45):32475–32489. doi: 10.1074/jbc.M113.514034

FIGURE 8.

FIGURE 8.

T1R2/T1R3 are dispensable for saccharin-suppressed lipolysis. A, 3T3-L1 adipocytes were treated for 1 h with Sacc, Fsk, or both. Glycerol concentration in the medium was measured before collection of protein lysates from control and treated cells. Lysates were then probed for pHSL by Western blotting (left panel). HSL phosphorylation was then quantified by densitometry (right panel). B, 3T3-L1 adipocytes were serum-starved for 2 h and pretreated with LY294002 (LY) for 1 h before a 2-h Sacc treatment. Glycerol secretion was then measured in medium. C, 3T3-L1 adipocytes were serum-starved for 2 h and then treated for 45 min with Fsk or Sacc. cAMP concentration was quantified by ELISA. T1R3 KO (D), T1R2 KO (E), or DKO (F) eMSC adipocytes were treated with Sacc, Fsk, or both for 4 h before measuring glycerol accumulation in medium. Significant differences p < 0.05, p < 0.01, and p < 0.005 are denoted *, **, and ***, respectively. Data are expressed as mean ± S.D. (error bars). Significance was determined using Student's t test. tHSL, total HSL.