FIGURE 1.

Thrombin modulates PAR1-PAR4 heterodimer formation. HEK293 cells were transfected with HA-PAR4-rLuc (0.03 μg) and V5-PAR1-GFP (0–2.5 μg) (A), HA-PAR1-rLuc (0.5 μg) and V5-PAR4-GFP (0–0.25 μg) (B), or HA-PAR1-rLuc (0.5 μg) and rhodopsin-GFP (0–1.5 μg) (C). Forty-eight hours post-transfection, the cells were unstimulated (solid circles) or treated with α-thrombin (10 nm) for 10 min (open circles). The cells were analyzed for GFP expression, luciferase activity, and BRET. The data are from three independent experiments in which all points were analyzed by global fit to hyperbolic or linear curve.