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Characterization of Sphk dKO macrophages. Efficiency of Sphk1 or Sphk2 deletion in isolated BMDMs was determined by PCR of genomic DNA (A), qRT-PCR of mRNA from BMDMs (B), and immunoblot analysis of BMDM extracts (C). Data are representative of at least three independent experiments. N/S, no signal detected. Data represent mean ± S.E. (error bars).
