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Ser-175 modification does not alter processivity. Run lengths of quantum dot cargos carried by a single kinesin were measured. A, for K888 mutants, we measured a run length of 1.06 ± 0.08 μm for S175A (red, n = 133) and 0.89 ± 0.08 μm for S175D (blue, n = 91). B, for full-length kinesin, we measured a run length of 0.97 ± 0.12 μm for unphosphorylated kinesin (green, n = 93) and 0.86 ± 0.11 μm for 80% phosphorylated kinesin (yellow, n = 81). Error bars are S.E.
