TABLE 1.

Summary of the kinetics of K888 mutants

Kinetics are from single-molecule motility studies and microtubule-activated ATPase assays of K888 mutants. The results from the motility studies are given in the left two columns. The microtubule-activated ATPase kinetics are in the right two columns. Errors are given as S.E.

Kinesin	KmATP	Vmax	KmMT	VmaxMT
	μm	nm/s	μm	ATP molecules/kinesin monomer/s
WT K888	35 ± 8	484 ± 23	0.70 ± 0.26	10.6 ± 1.4
S175A K888	44 ± 9	493 ± 24	0.65 ± 0.25	10.8 ± 1.1
S175D K888	44 ± 10	494 ± 28	0.74 ± 0.22	11.3 ± 1.6