TABLE 2.
Distances and velocities of beads coated with both kinesin and dynein
Velocities and total distances traveled of dynein and kinesin coated beads are shown. Top three rows correspond to datasets with K888 and dynein. Bottom two rows correspond to datasets with full-length kinesin and dynein. Errors are S.D.
Mixture of motors | Number of observations | Net displacement | Velocity |
---|---|---|---|
μm | nm/s | ||
WT (K888) + dynein | 93 | 0.0 ± 3.6 | −22.2 ± 192 |
S175A (K888) + dynein | 74 | 0.4 ± 3.8 | 23.5 ± 146 |
S175D (K888) + dynein | 151 | −1.7 ± 4.3 | −55.6 ± 127 |
Unphosphorylated kinesin (full-length) + dynein | 105 | 3.4 ± 4.0 | 169.8 ± 251 |
80% phosphorylated kinesin (full-length) + dynein | 117 | 0.6 ± 4.4 | 51.7 ± 210 |