TABLE 3.
Primers used in this study
| Primers | 5′ → 3′ | Notes |
|---|---|---|
| E102A-F | gatacctgatccacgcgttcgacccgtggtt | Mutagenesis |
| E102A-R | aaccacgggtcgaacgcgtggatcaggtatc | Mutagenesis |
| D104A-F | ctgatccacgagttcgccccgtggttcaactac | Mutagenesis |
| D104A-R | gtagttgaaccacggggcgaactcgtggatcag | Mutagenesis |
| D223N-F | atggcgggtgagttcaacaacgagtgcatcg | Mutagenesis |
| D223N-R | cgatgcactcgttgttgaactcacccgccat | Mutagenesis |
| E225Q-F | gtgagttcgacaaccagtgcatcgccgtc | Mutagenesis |
| E225Q-R | gacggcgatgcactggttgtcgaactcac | Mutagenesis |
| D639A-F | ttggcctggtgggcctacggctaccag | Mutagenesis |
| D639A-R | ctggtagccgtaggcccaccaggccaa | Mutagenesis |
| D698A-F | gggcagagcggcgccctgatgaagtca | Mutagenesis |
| D698A-R | tgacttcatcagggcgccgctctgccc | Mutagenesis |
| K701A-F | gcggcgacctgatggcgtcaccgcacatgg | Mutagenesis |
| K701A-R | ccatgtgcggtgacgccatcaggtcgccgc | Mutagenesis |
| Ost3-Inf-F | gaattcgagctcggtacccggggatccgatgtcattcccggcggctc | Cloning |
| Ost3-Inf-R | aagcttgcatgcctgcaggtcgactggactaggcagcctcacaaa | Cloning |
| Ost6-Inf-F | gaattcgagctcggtacccggggatcctgtctgagttaggagccaac | Cloning |
| Ost6-Inf-R | aagcttgcatgcctgcaggtcgacttcattcctgtgtgatttaa | Cloning |
| YEp352-Inf-F | gtcgacctgcaggcatgc | Cloning |
| YEp352-Inf-R | ggatccccgggtaccgag | Cloning |
| ScSTT3-Inf-F | gggctgcaggaattcatgggatccgaccggtcg | Cloning |
| ScSTT3-Inf-R | ggtatcgataagcttttagactctcaagcctaa | Cloning |
| p425GPD-Inf-F | aagcttatcgataccgtc | Cloning |
| p425GPD-Inf-R | gaattcctgcagcccggg | Cloning |