TABLE 4.
Quantitation of the PA-labeled fOSs from png1Δ ams1Δ cells
| Glycan IDa | Amountsb |
Number of hexoses shifted after jack bean α-mannosidase digestionc | Number of hexoses shifted after α1,2-mannosidase digestiond | Number of hexoses shifted after α1,2-mannosidase and α1,6-mannosidase double digestione | Glucose unitg | |
|---|---|---|---|---|---|---|
| png1Δ ams1Δ | ams1Δ | |||||
| pmol/100 A600 | ||||||
| a | 2.0 | 134.8 | 6 | 2 | NDf | 5.76 |
| b-1 | 9.1 | 167.4 | 7 | 3 | ND | 4.91 |
| b-2 | 1.1 | 45.1 | 7 | 2 | 3 | 5.75 |
| c | 1.6 | 28.6 | 8 | 3 | 4 | 4.86 |
| Total amounts | 13.8 | 375.9 | ||||
a Glycan ID was based on Fig. 1B.
b Amounts of the fOSs were estimated from peak area of PA-Glc6 in dual-gradient, reversed-phase HPLC (14).
c PA-fOSs were isolated in dual-gradient, reversed-phase HPLC (14) and digested with jack bean α-mannosidase. The digests were analyzed by size fractionation HPLC.
d PA-fOSs were isolated in dual-gradient, reversed-phase HPLC (14) and digested with α1,2-mannosidase. The digests were analyzed by size fractionation HPLC.
e PA-fOSs were isolated in dual-gradient, reversed-phase HPLC (14) and double digested with α1,2-mannosidase and α1,6-mannosidase. The digests were analyzed by size fractionation HPLC.
f ND, not done.
g Glucose units of the fOSs were determined by dual-gradient, reversed-phase HPLC (14).