TABLE 1.

List of PCR primers

PCR were performed with primer pairs amplifying short fragments for each gene. Primer pairs were custom-designed to efficiently anneal to homologous nucleotide sequences from rat.

Gene	Primer sequence	Product size	Annealing temperature
CB1R	Forward: 5′-CGTCGTTCAAGGAGAATGAGG-3′	213 bp	57 °C
	Reverse: 5′-TGCCGATGAAGTGGTAGGAAG-3′
CB2R	Forward: 5′-GAGTGGAGAGATCCGCTCTG-3′	93 bp	57 °C
	Reverse: 5′-GGGGCTTCTTCTTTCCCCTC-3′
TRPV1	Forward: 5′-AGCGAGTTCAAAGACCCAGA-3′	233 bp	57 °C
	Reverse: 5′-TTCTCCACCAAGAGGGTCAC-3′
GPR55	Forward: 5′-ACGGGAGTGTCTTCACCATC-3′	173 bp	57 °C
	Reverse: 5′-CACTCCCCTGTGGAAAGTGT-3′
DAGLα	Forward: 5′-ACCTGCGGCATCGGTTAGAG-3′	81 bp	57 °C
	Reverse: 5′-TTGTCCGGGTGCAACAGAG-3′
DAGLβ	Forward: 5′-CGAGCTGCCCTTCATAGTGG-3′	81 bp	57 °C
	Reverse: 5′-TCCTGGAGAGACATGGTCCC-3′
FAAH	Forward: 5′-TGGAAGTCCTCCAAGAGCCCA-3′	197 bp	57 °C
	Reverse: 5′-TGTCCATAGACACAGCCCTTCAG-3′
MGL	Forward: 5′-ACCAACCCACTTTTCTGGCA-3′	170 bp	57 °C
	Reverse: 5′-CAACCTCCGACTTGTTCCGA-3′
NAPE-PLD	Forward: 5′-CGTGCTCAGATGGCTGATAA-3′	192 bp	57 °C
	Reverse: 5′-ATGAGCTCGTCCATTTCCAC-3′