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. 2013 Oct 2;288(45):32700–32707. doi: 10.1074/jbc.M113.506329

FIGURE 3.

FIGURE 3.

Schematic image of the rotation assay. Shown is the experimental setup for single-molecule rotation assay of reconstituted EhV1. The stator A3B3 ring of EhV1 was fixed on the glass surface with His6 tag at the N termini of the A subunits. Streptavidin-coated 40-nm gold colloid was attached to biotinylated cysteine residues in the rotor DF subunit.