FIGURE 3.
Schematic image of the rotation assay. Shown is the experimental setup for single-molecule rotation assay of reconstituted EhV1. The stator A3B3 ring of EhV1 was fixed on the glass surface with His6 tag at the N termini of the A subunits. Streptavidin-coated 40-nm gold colloid was attached to biotinylated cysteine residues in the rotor DF subunit.