FIGURE 4.

Two distinct states in the rotation of EhV₁. A and D, time courses of rotation, including two reversible states of single molecule-reconstituted EhV₁ (3 mm ATP) and AviTag-EhV₁ (2 mm ATP). The rotations in the clear and unclear states are highlighted in red and blue, respectively. B and E, The x-y trajectories of the centroid of a rotating gold colloid shown in A and D. C and F, distributions of rotary angles shown in A and D. The numbers in A–C and D–F indicate the corresponding parts. G and H, distributions of duration times of clear and unclear states of reconstituted EhV₁. The data at various ATP concentrations (from 10 μm to 40 mm) were analyzed collectively. The bin width was 0.1 s. The solid curves show the fit with single-exponential decay: constant × (exp(−t/τ)), where τ = 0.13 ± 0.003 s (mean ± S.E. of fitting, 238 events from 58 molecules) and 0.33 ± 0.009 s (mean ± S.E., 199 events from 58 molecules) for the clear (G) and unclear (H) states, respectively.