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. 2013 Oct 2;288(45):32700–32707. doi: 10.1074/jbc.M113.506329

TABLE 1.

Kinetic parameters of V1-ATPases and F1-ATPases from different sources

Protein/measurement temperature kon(ATP)a kon(ATP) (3 × Vmax/Km)a τ12a Ref.
m1 s1 m1 s1 ms
EhV1d
    26 ± 1 °C (2.3 ± 0.03) × 106 (2.2 ± 0.4) × 106 2.6 ± 0.1/0.5 ± 0.02e This work

TtV1
    23 °C 1.5 × 106 0.84 × 106 2.8/2.8 11
    24 ± 1 °C 1.39 × 106 21

TF1
    23 °C 3.0 × 107 2.6 × 107 1.6/0.71 13
    25 ± 1 °C 1.34/0.29 20
    25 ± 1 °C 2.2 × 107 27

EF1
    23 °C 4.7 × 107 6.4 × 107 0.41/0.29 15

a The second-order binding rate constant for ATP (kon(ATP)) was determined from the distribution of the duration of the ATP-waiting pause.

b The second-order binding rate constant for ATP (kon(ATP)) was determined from 3 × Vmax/Km.

c Time constants were determined from the distribution of the duration of the catalytic pause, which corresponds to ATP cleavage and either ADP or phosphate release (or both).

d The values are the mean ± S.E. of fitting.

e The values were obtained at 40 mm ATP.