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. 2013 Sep 26;288(45):32731–32741. doi: 10.1074/jbc.M113.503771

FIGURE 3.

FIGURE 3.

In vitro invasion of MCF10CA1a cells after siRNA treatment. A, mRNA levels of a subunit isoforms in MCF10CA1a cells after siRNA treatment. Cells were exposed to a subunit isoform-specific siRNA pools for 96 h prior to measuring mRNA levels, as described under “Experimental Procedures.” Quantitative RT-PCR was conducted as described in the legend for Fig. 2. Knockdown is reported as the ratio of a subunit isoform mRNA in cells treated with siRNA versus a subunit isoform mRNA in untreated cells. Values are the mean of three separate experiments. Error bars indicate S.D. B, Cells were exposed to a subunit isoform-specific siRNAs for 81 h prior to measuring invasion through MatrigelTM-coated FluoroBlokTM wells. Invasion is reported as the percentage of invasion observed for siRNA-treated cells relative to untreated cells (Mock). Three wells were counted per sample, with 15 images analyzed per well. Values are the mean ± S.D. of two or three independent experiments. *, p < 0.005.