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. 2013 Sep 30;288(45):32766–32776. doi: 10.1074/jbc.M113.511303

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Neddylation of Cul1 is not required for assembly of SCF^Fbxl3. A, lysates of HeLa cells expressing FLAG-tagged Fbxl3 as well as HA-tagged forms of Cry1, Cul1, or Cul1(K720R) were subjected to immunoprecipitation (IP) with anti-FLAG, and the resulting precipitates, as well as the original cell lysates (Input), were subjected to immunoblot analysis with antibodies to the indicated proteins. B, HeLa cells expressing FLAG-tagged Fbxl3 and HA-tagged Cry1 were treated with 1 μm NAE inhibitor for 24 h, lysed, and subjected to immunoprecipitation with anti-FLAG. The resulting precipitates and original cell lysates were subjected to immunoblot analysis with antibodies to the indicated proteins.