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. 2013 Sep 30;288(45):32766–32776. doi: 10.1074/jbc.M113.511303

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Fbxl3 is more stable than other F-box proteins. HeLa cells expressing FLAG-tagged forms of Fbxl3 (A), Skp2 (B), Fbxw7α (C), or Fbxo31 (D) were exposed to cycloheximide (CHX) for the indicated times in the presence of NAE inhibitor, MG132, or dimethyl sulfoxide (DMSO) vehicle, after which cell lysates were subjected to immunoblot analysis with anti-FLAG (left panels). The percentage of each FLAG-tagged F-box protein remaining after the various chase times was determined (right panels, n = 3, means ± S.D. (error bars)). The half-life (t_1/2) of each F-box protein is indicated.