Fig. 1.

Sperm purification and overview of shotgun proteomics analyses. A, Epi-fluorescent/DIC image of purified macaque sperm. DAPI fluorescence in bright oblate spheroids (white, arrow) indicate the presence of DNA in morphologically normal sperm nuclei and head structures. Associated sperm midpiece (bracket) and flagella (arrowheads) were visualized simultaneously under Nomarski DIC optics also indicative of normal morphology for mature sperm. Bar = 10 microns. B, Venn diagram of protein overlap in three sperm samples identified using shotgun MS/MS.