Fig. 3.

pUL46 associates with viral and host proteins following HSV-1 infection. A, Schematic of proteomic workflow for identification of specific interacting partners of pUL46. B, Spectral count comparison for pUL46 interactions observed using mild and stringent isolation buffer conditions. Viral and host proteins with SAINT probability scores greater than 0.9 were considered likely specific, and the ratios of spectral counts in the stringent versus mild conditions were plotted on a log₂ scale. Select functional clusters of host and viral proteins are illustrated with circles and diamonds, respectively.