Table I. Identification of pUL46 phosphorylation during HSV-1 infection. Twenty-three total modifications are listed, including 17 previously unreported sites. All spectra were manually inspected to ensure correct site localization. Sites of ambiguity with two adjacent potential phosphosites are indicated by parentheses.

Phosphosite(s)	Sequence(s)	z	Xc	PEP	phosphoRS (%)	Δppm	Reference
T18	CLtPANLIR	2	3.56	2.60E-05	T(3): 100.0	−4.53	This study
S263	FLPLGGsPEAPAETFAR	2	5.21	2.30E-08	S(7): 100.0	−3.29	This study
T431 (or T430)	RTtWSAGPPPDDMASGPGGHR	3	5.61	1.20E-05	T(3): 92.6; T(2): 6.9	0.28	This study
S433	TTWsAGPPPDDMASGPGGHR	3	3.34	2.40E-06	S(4): 99.7	−4.09	This study
T431/S433	RTtWsAGPPPDDMASGPGGHR	3	5.05	8.80E-10	T(3): 94.6; S(5): 100.0	−3.99	This study
S443	TTWSAGPPPDDMAsGPGGHR	3	3.32	6.60E-04	S(14): 100.0	−4.35	This study
S478	LHsTPASTR	2	2.66	1.50E-02	S(3): 99.2	1.17	21
S478/T479	LHstPASTR	2	3.51	1.70E-03	S(3): 100.0; T(4): 100.0	0.09	This study
S478/S481	LHsTPAsTR	2	3.2	4.90E-02	S(3): 92.1; S(7): 92.0	−4.01	This study
T545	RPPAAtCPLLVR	3	2.38	1.80E-04	T(6): 100.0	−3.35	This study
S554	RAsLGSLDRPR	2	2.33	8.90E-02	S(3): 99.9	−0.88	21
S554/S557	RAsLGsLDRPR	2	2.98	2.30E-02	S(3): 100.0; S(6): 100.0	−2.94	This study
Y579 (or T578)	VWGPAPEGEPDQMEATyLTADDDDDDAR	3	5.26	1.50E-06	Y(17): 99.3	−0.96	This study
T581	VWGPAPEGEPDQMEATYLtADDDDDDAR	3	6.21	7.00E-15	T(19): 100.0	−0.47	This study
S611	HAPYEDDEsIYETVSEDGGR	3	4.28	1.80E-05	S(9): 98.6	−0.59	This study
Y613	HAPYEDDESIyETVSEDGGR	3	4.76	4.20E-09	Y(11): 100.0	0.42	This study
T615	HAPYEDDESIYEtVSEDGGR	3	4.27	1.20E-06	T(13): 99.9	−0.52	This study
S617	HAPYEDDESIYETVsEDGGR	3	5.58	2.00E-07	S(15): 100.0	0.34	21
Y606/S617	HAPyEDDESIYETVsEDGGR	3	4.33	1.70E-03	Y(4): 99.3; S(15): 100.0	−3.65	This study
S611/S617	HAPYEDDEsIYETVsEDGGR	3	5.61	2.80E-07	S(9): 100.0; S(15): 100.0	−0.33	This study
Y613/S617	HAPYEDDESIyETVsEDGGR	3	4.76	3.30E-08	Y(11): 100.0; S(15): 100.0	1.03	This study
T615/S617	HAPYEDDESIYEtVsEDGGR	3	3.64	3.00E-04	T(13): 100.0; S(15): 100.0	0.5	This study
Y606/T615/S617	HAPyEDDESIYEtVsEDGGR	3	4.32	7.50E-04	Y(4): 92.2; S(9): 7.7; T(13): 100.0; S(15): 100.0	−1.33	This study
S611/Y613/S617	HAPYEDDEsIyETVsEDGGR	3	5.37	2.00E-05	S(9): 100.0; Y(11): 100.0; S(15): 100.0	−2.2	This study
Y624	VyEEIPWMR	2	3.08	6.00E-05	Y(2): 100.0	−3.94	This study
S647	VYENVCVNTANAAPAsPYIEAENPLYDWGGSALFSPPGR	3	6.71	3.60E-08	S(16): 89.2; Y(18): 10.8	−1.6	This study
S666	VYENVCVNTANAAPASPYIEAENPLYDWGGSALFsPPGR	4	4.08	5.00E-05	S(35): 100.0	−0.7	This study
S679	TGPPPPPLsPSPVLAR	3	3.3	7.60E-05	S(9): 100.0	0.06	21
S681	TGPPPPPLSPsPVLAR	2	4.11	6.00E-06	S(11): 100.0	−0.04	21
S679/S681	TGPPPPPLsPsPVLAR	3	4.15	2.90E-07	S(9): 100.0; S(11): 100.0	−4.02	This study