Fig. 3.

Amino acid distribution (WebLogo) and iceLogo analysis of the potential PKA substrates and their overlap in different phosphorylation datasets. The dataset used to create the frequencies in logo (A) and iceLogo (B) contained phosphopeptides with a PKA phosphosite localized with a probability of >0.75 (pRS probability). C, Venn diagrams representing the overlap between the very large unbiased Jurkat (phospho)proteome dataset reported by Mayya et al. and data from this study with a specific enrichment protocol. The overlap at the phosphoprotein level (top) is still reasonable, but at the phosphopeptide level (bottom) it is extremely low.