Skip to main content
. 2013 Jul 23;12(11):3350–3359. doi: 10.1074/mcp.O113.028456

Fig. 4.

Fig. 4.

Quantitative analysis of the PGE2-regulated phosphoproteome. Density curves of observed peptide ratios before (MIX) and after (IP) the immunoprecipitation show that the three differentially stable-isotope dimethyl-labeled samples were mixed as 1:1:1, with negligible nonphosphopeptides with a large dimethyl ratio between stimulated and unstimulated cells. Density curves of the quantified phosphopeptides, with or without a localized putative PKA motif, show a general increase in the level of phosphorylation upon PGE2 stimulation after 1 min (A) and 60 min (B).

HHS Vulnerability Disclosure