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N-terminal processing and its effect on in vivo protein stability. A, enrichment scores of mutations in codon 2. B, spotting assay of the N2 mutants with the highest log2E scores identified in Fig. 5A. Changes to Lys or Arg resulted in good growth on the -Leu -Ura plate. C, effect of the nat3Δ allele on Deg1–Leu2M1Δ production. BY4741 and nat3Δ strains carrying Deg1–Leu2M1Δ variants were spotted on control and selection plates.
