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. 2013 Aug 13;12(11):3431–3442. doi: 10.1074/mcp.M112.025213

Fig. 1.

Fig. 1.

Efficiency of Rubisco removal by PEI (Polyethyleneimine) precipitation. A, PEI precipitation of total soluble protein isolated by soluble protein extraction buffer. From the left to the right, lane 1 shows the total soluble protein extracted by soluble protein extraction buffer. Lane 2 to 5 shows the supernatant of soluble protein after precipitation with 10, 25, 50, and 100 mg/g PEI (weight/total protein), respectively. Lane 6 and 7 are dissolved protein pellets after PEI precipitation. B, Incompatibility between PEI precipitation and total protein extracted by Sigma Aldrich Plant Total Protein Extraction kit. From the left to the right, lane 1 shows the total protein extracted by the kit. Lane 2 and 3 shows total protein supernatant after PEI precipitation at 25 and 50 mg/g, respectively. PEI concentration (mg/g) is as indicated at the top. Molecular weight markers in kDa are shown on the left.