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qRT-PCR validation of mRNA expression for selected differentially expressed proteins. qRT-PCR validation of (A) Os12g14440 and (B) Os03g48770 gene expression after 0 h, 12 h, 24 h, and 48 h treatments. The relative expression levels as compared with the lowest expressing sample in the group were derived from modified ΔΔCT method. The average ratio and 95% confidence interval were derived from triplicate assays.
