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. 2013 Oct 18;8(10):e78031. doi: 10.1371/journal.pone.0078031

Figure 1. Validation of microarray predicted RBM38-regulated splicing events.

Figure 1

A) Semi-quantitative RT-PCR analysis of HJAY microarray targets in MCF-7 cells. Average percentages of exon inclusion with standard deviations compiled from three experiments are indicated below a representative gel. . For AP1G2, only two replicates were included in analyses. B) Western blot detection of siRNA knockdown of RBM38 in MCF-7 cell line (upper panel). Actin is shown as a loading control (lower panel). C) Bar graph representing data from panel A. siControl and siRBM38 treated cells are shown in light and dark gray bars, respectively (P-values of ≤ 0.05 are annotated with an asterisk).