Figure 1. Depletion of inflammatory monocytes during the second week of M. tuberculosis infection abrogates transport of live bacteria to mLNs and increases pulmonary bacterial burden.
(A) CFU plots from the lungs of CCR2-DTR mice receiving DT or PBS on days −1, 0 and 1 and harvested on day 7. (B) CFU plots of the lungs of CCR2-DTR mice receiving DT or PBS on days 7, 9 and 11 and harvested on day 15 post infection. CFU counts from the lungs (C) and mLN (D) of CCR2-DTR mice given DT treatment on days 7, 9 and 11 and harvested on day 12. Each dot represents an individual mouse. Error bars denote SEM. Data are representative of two independent experiments.
Figure 1—figure supplement 1. DT administration depletes IMs from the lungs and mLNs of infected mice.
CCR2-DTR mice received PBS or DT on days 7, 9 and 11 post infection and were harvested on day 12 when flow cytometry was performed to quantify the IM population in the lungs (A) and mLN (B). Data are representative of three independent experiments.