Figure 5.

EV71-infected CD14+ cells induced a specific anti-EV71 immune response in monkeys. The CD14⁺ cells from three normal monkeys were infected by EV71 (MOI=1) in vitro. After 24 h p.i., EV71-infected CD14⁺ cells were transfused either back into the same monkeys or into three non-donor monkeys in experimental group 2. PBMCs were collected from the transfused monkeys at 5 days post-transfusion. The percentages of cells producing IFN-γ in the sera of monkeys were determined using CFC as described in the section on ‘Materials and methods'. P value vs. positively and negatively stimulated group. Three independent experiments were performed. CFC, cytokine flow cytometry.