Fig. 2.

PGG suppresses IL-4-induced STAT6 activation by inhibiting the IL-4 signal pathway. DND39 cells were stimulated with IL-4 (250 U/ml) in the presence of 25 μM of PGG for 10 min or 30 min. Lysates were then immunoprecipitated with anti-JAK3, anti-IL-4Rα, or anti-STAT6 antibodies and analyzed by western blotting using anti-phosphotyrosine antibody (4G10) for phosphorylated JAK3 and IL-4Rα or anti-phosphotyrosine antibody (PY20) for phosphorylated STAT6. Each experiment was repeated three times in triplicate.