Fig. 3.

PGG specifically inhibits IL-4 and IL-13 signaling, but not IFN-γ signaling. (A) DND39 cells were treated with IFN-γ (400 ng/ml) and PGG for 30 min. Lysates were then immunoprecipitated with anti-STAT1 antibody and analyzed by western blotting using an anti-pTyr-STAT1 antibody. (B) NIH-3T3 cells were treated with IL-13 (10 ng/ml) and PGG (50 μM) for 30 min. Lysates were then immunoprecipitated with anti-STAT6 antibody and analyzed by western blotting using an anti-phosphotyrosine antibody (PY20). Each experiment was repeated three times in triplicate.