Fig. 1.

Geldanamycin dependent degradation of GR in WT, Ubr1^−/− and Chip^−/− MEF cells. (A) WT, Ubr1^−/− and CHIP^−/− MEF cells were treated with 100 nM of GA for indicated times. 40 μg of total protein from each cell line were analyzed by SDS–PAGE and probed with anti-GR; PI3K was used as a loading control. (B) WT, Ubr1^−/− and CHIP^−/− MEF cells were treated with different concentration of GA for 6 hours. 40μg of total protein from each cell line were fractionated by SDS–PAGE and probed with anti-GR. PI3K levels were analyzed as a loading control.