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. 2013 Aug 2;3:346–351. doi: 10.1016/j.fob.2013.07.007

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© 2013 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig. 3 — Double reciprocal analysis of PG545 inhibition of heparanase (panel A). Fondaparinux assays conducted as per Section 2. Data are means of 2 measurements. Lines were generated from the apparent K_m and V_max values. The slopes from panel A were replotted as a function of PG545 concentration in panel B. The solid line in panel B is the fit of Eq. (4) to the data (R² = 0.993, SS = 0.735) and the dotted line is the fit of Eq. (2) (R² = 0.950, SS = 5.450) for comparison.