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Kinetic constants for pyruvate reduction and lactate oxidation reactions of LDHA. Kinetic constants were determined in 0.1 M Tris–maleate (pH 7.0) with 1 mM FBP at 30 °C.
| Substrate | Km value (mM) | Vm value (μmol min−1 mg−1) |
|---|---|---|
| NADH | 0.066 ± 0.008 | 1534 ± 45 |
| Pyruvate | 1.95 ± 0.12 | |
| NAD | 0.38 ± 0.01 | 313 ± 3 |
| l-Lactate | 158 ± 4 |
